16S rRNA gene-based analysis of fecal microbiota from preterm infants with and without necrotizing enterocolitis.

ABSTRACT: Neonatal necrotizing enterocolitis (NEC) is an inflammatory intestinal disorder affecting preterm infants. Intestinal bacteria have an important function; however no causative pathogen has been identified. The purpose of this study was to determine if there are differences in microbial patterns that may be critical to the development of this disease. Fecal samples from 20 preterm infants, 10 with NEC and 10 matched controls (including 4 twin pairs) were obtained from patients in a single site level III neonatal intensive care unit. Bacterial DNA from individual fecal samples was PCR-amplified and subjected to terminal restriction fragment length polymorphism analysis and library sequencing of the 16S rRNA gene to characterize diversity and structure of the enteric microbiota. The distribut

SUBMITTER: Wang Y 

PROVIDER: S-EPMC2713796 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature