Expressional dynamics of minisatellite 33.15 tagged spermatozoal transcriptome in Bubalus bubalis.

ABSTRACT:

Background

Transcriptionally quiescent spermatozoa have been established to be a repository of mRNA coding for several functionally essential cellular proteins. This entourage of mRNA is envisaged to be involved in post-fertilization and early embryogenesis. Minisatellites tagged with mRNA transcripts have been implicated with gene organization, regulation and function. However, the organization and expression of the minisatellite tagged transcript diversity, particularly in spermatozoa, remains unclear.

Results

In the present study, we identified and characterized 12 mRNA transcripts from the spermatozoa of water buffalo Bubalus bubalis employing minisatellite associated sequence amplification (MASA) and a consensus sequence of 33.15 repeat loci. Of these 33.15 tagged trans

SUBMITTER: Srivastava J 

PROVIDER: S-EPMC2713999 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature