Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library.

ABSTRACT:

Background

Drought is one of the most important environmental factors causing water stress for cotton, and it greatly limits cotton growth and crop productivity. So far only a few drought-tolerance genes have been functionally characterized in details, and most efforts on this topic have been made in model organisms. Therefore, to identify more drought-related genes in cotton plays a crucial role in elucidating the underlying mechanisms of drought tolerance as well as utilizing bioengineering techniques to improve the tolerance in this organism.

Findings

Here we constructed a subtractive drought-tolerance cDNA library using suppressive subtractive hybridization (SSH). Through differential screening and bioinformatics analysis, we identified 392 positive clones with different

SUBMITTER: Zhang L 

PROVIDER: S-EPMC2714314 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature