Integrins are required for the differentiation of visceral endoderm.

ABSTRACT: Integrins of the beta1 subfamily are highly expressed in the early mouse embryo and are essential for the formation of primitive germ layers from the inner cell mass (ICM). We investigated the mechanisms by which alphabeta1 integrins regulate ICM morphogenesis by using the embryonic-stem-cell-derived embryoid body (EB), a model for peri-implantation development. Ablation of integrin beta1 in EBs resulted in endoderm detachment and in maturation defects, which were manifested by the mislocalization of GATA4 in the cytoplasm and the markedly reduced synthesis of basement membrane (BM) components and the lineage marker disabled homolog 2. The mutant endoderm cells failed to spread on BM substrates, but could spread on vitronectin, which induced upregulation of alphavbeta3 integrin and integri

SUBMITTER: Liu J 

PROVIDER: S-EPMC2714419 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature