Reactivation from latency displays HIV particle budding at plasma membrane, accompanying CD44 upregulation and recruitment.

ABSTRACT:

Background

It has been accepted that HIV buds from the cell surface in T lymphocytes, whereas in macrophages it buds into intracellular endosomes. Recent studies, on the other hand, suggest that HIV preferentially buds from the cell surface even in monocytic cells. However, most studies are based on observations in acutely infected cells and little is known about HIV budding concomitant with reactivation from latency. Such studies would provide a better understanding of a reservoir for HIV.

Results

We observed HIV budding in latently infected T lymphocytic and monocytic cell lines following TNF-alpha stimulation and examined the upregulation of host factors that may be involved in particle production. Electron microscopy analysis revealed that reactivation of latently infect

SUBMITTER: Suyama M 

PROVIDER: S-EPMC2714482 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature