Deep sampling of the Palomero maize transcriptome by a high throughput strategy of pyrosequencing.

ABSTRACT:

Background

In-depth sequencing analysis has not been able to determine the overall complexity of transcriptional activity of a plant organ or tissue sample. In some cases, deep parallel sequencing of Expressed Sequence Tags (ESTs), although not yet optimized for the sequencing of cDNAs, has represented an efficient procedure for validating gene prediction and estimating overall gene coverage. This approach could be very valuable for complex plant genomes. In addition, little emphasis has been given to efforts aiming at an estimation of the overall transcriptional universe found in a multicellular organism at a specific developmental stage.

Results

To explore, in depth, the transcriptional diversity in an ancient maize landrace, we developed a protocol to optimize the sequenc

SUBMITTER: Vega-Arreguin JC 

PROVIDER: S-EPMC2714558 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature