Gene expression profiles of acute exacerbations of idiopathic pulmonary fibrosis.

ABSTRACT:

Rationale

The molecular mechanisms underlying acute exacerbations of idiopathic pulmonary fibrosis (IPF) are poorly understood. We studied the global gene expression signature of acute exacerbations of IPF.

Objectives

To understand the gene expression patterns of acute exacerbations of IPF.

Methods

RNA was extracted from 23 stable IPF lungs, 8 IPF lungs with acute exacerbation (IPF-AEx), and 15 control lungs and used for hybridization on Agilent gene expression microarrays. Functional analysis of genes was performed with Spotfire and Genomica. Gene validations for MMP1, MMP7, AGER, DEFA1-3, COL1A2, and CCNA2 were performed by real-time quantitative reverse transcription-polymerase chain reaction. Immunohistochemistry and in situ terminal deoxynucleotidyltransferase dU

SUBMITTER: Konishi K 

PROVIDER: S-EPMC2714820 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature