In silico identification of a core regulatory network of OCT4 in human embryonic stem cells using an integrated approach.

ABSTRACT:

Background

The transcription factor OCT4 is highly expressed in pluripotent embryonic stem cells which are derived from the inner cell mass of mammalian blastocysts. Pluripotency and self renewal are controlled by a transcription regulatory network governed by the transcription factors OCT4, SOX2 and NANOG. Recent studies on reprogramming somatic cells to induced pluripotent stem cells highlight OCT4 as a key regulator of pluripotency.

Results

We have carried out an integrated analysis of high-throughput data (ChIP-on-chip and RNAi experiments along with promoter sequence analysis of putative target genes) and identified a core OCT4 regulatory network in human embryonic stem cells consisting of 33 target genes. Enrichment analysis with these target genes revealed that this i

SUBMITTER: Chavez L 

PROVIDER: S-EPMC2714862 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature