Phytochemistry of cimicifugic acids and associated bases in Cimicifuga racemosa root extracts.

ABSTRACT:

Introduction

Earlier studies reported serotonergic activity for cimicifugic acids (CA) isolated from Cimicifuga racemosa. The discovery of strongly basic alkaloids, cimipronidines, from the active extract partition and evaluation of previously employed work-up procedures has led to the hypothesis of strong acid/base association in the extract.

Objective

Re-isolation of the CAs was desired to permit further detailed studies. Based on the acid/base association hypothesis, a new separation scheme of the active partition was required, which separates acids from associated bases.

Methodology

A new 5-HT(7) bioassay guided work-up procedure was developed that concentrates activity into one partition. The latter was subjected to a new two-step centrifugal partitioning chromat

SUBMITTER: Godecke T 

PROVIDER: S-EPMC2715282 | biostudies-literature | 2009 Mar-Apr

REPOSITORIES: biostudies-literature