Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro.

ABSTRACT:

Background

Infectious salmon anemia virus (ISAV) is an aquatic orthomyxovirus and the causative agent of infectious salmon anemia (ISA), a disease of great importance in the Atlantic salmon farming industry. In vitro, ISAV infection causes cytophatic effect (CPE) in cell lines from Atlantic salmon, leading to rounding and finally detachment of the cells from the substratum. In this study, we investigated the mode of cell death during in vitro ISAV infection in different Atlantic salmon cell lines, using four ISAV strains causing different mortality in vivo.

Results

The results show that caspase 3/7 activity increased during the course of infection in ASK and SHK-1 cells, infected cells showed increased surface expression of phosphatidylserine and increased PI uptake, compare

SUBMITTER: Schiotz BL 

PROVIDER: S-EPMC2715388 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature