Large-scale production and study of a synthetic G protein-coupled receptor: human olfactory receptor 17-4.

ABSTRACT: Although understanding of the olfactory system has progressed at the level of downstream receptor signaling and the wiring of olfactory neurons, the system remains poorly understood at the molecular level of the receptors and their interaction with and recognition of odorant ligands. The structure and functional mechanisms of these receptors still remain a tantalizing enigma, because numerous previous attempts at the large-scale production of functional olfactory receptors (ORs) have not been successful to date. To investigate the elusive biochemistry and molecular mechanisms of olfaction, we have developed a mammalian expression system for the large-scale production and purification of a functional OR protein in milligram quantities. Here, we report the study of human OR17-4 (hOR17-4) pur

SUBMITTER: Cook BL 

PROVIDER: S-EPMC2715541 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature