Extended-spectrum properties of CMY-30, a Val211Gly mutant of CMY-2 cephalosporinase.

ABSTRACT: CMY-30, a Val211Gly mutant of CMY-2 cephalosporinase, was derived by mutagenesis. The hydrolytic efficiency of CMY-30 against expanded-spectrum cephalosporins was higher than that of CMY-2 due to increased k(cat) values. Findings indicate a role of the Omega loop residue 211 in determining the substrate specificities of CMYs also corroborated by modeling studies.

SUBMITTER: Kotsakis SD 

PROVIDER: S-EPMC2715598 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature