Symbiosis-specific expression of Rhizobium etli casA encoding a secreted calmodulin-related protein.

ABSTRACT: Symbiosis between Rhizobium and its leguminous host requires elaborate communication between the partners throughout the interaction process. A calmodulin-like protein, termed calsymin, was identified in Rhizobium etli; a calmodulin-related protein in a Gram-negative bacterium had not been described previously. Calsymin possesses three repeated homologous domains. Each domain contains two predicted EF-hand Ca(2+)-binding motifs. Ca(2+)-binding activity of calsymin was demonstrated on purified protein. R. etli efficiently secretes calsymin without N-terminal cleavage of the protein. The gene encoding calsymin, casA, is exclusively expressed during colonization and infection of R. etli with the host. Expression of casA is controlled by a repressor protein, termed CasR, belonging to the TetR

SUBMITTER: Xi C 

PROVIDER: S-EPMC27157 | biostudies-literature | 2000 Sep

REPOSITORIES: biostudies-literature