Abundant expression of ponsin, a focal adhesion protein, in lens and downregulation of its expression by impaired cytoskeletal signaling.

ABSTRACT:

Purpose

This study was undertaken to improve understanding of the defective lens developmental changes induced by the transgenic overexpression of the Rho GDP dissociation inhibitor RhoGDIalpha. The study was focused on a single differentially expressed gene encoding ponsin, a cell adhesion interacting signaling adaptor protein.

Methods

Total RNA extracted from the P7 lenses of Rho GDIalpha transgenic mice was subjected to cDNA microarray analysis. Ponsin distribution in the mouse lenses was determined by immunofluorescence and immunoblot analyses. Interactions among ponsin, actin, and Rho GTPase signaling pathways were explored in lens epithelial cells.

Results

The RhoGDIalpha transgenic mouse lenses revealed a marked downregulation of expression of multiple splice v

SUBMITTER: Rao PV 

PROVIDER: S-EPMC2716002 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature