Versatile online-offline engine for automated acquisition of high-resolution tandem mass spectra.

ABSTRACT: For automated production of tandem mass spectrometric data for proteins and peptides >3 kDa at >50 000 resolution, a dual online-offline approach is presented here that improves upon standard liquid chromatography-tandem mass spectrometry (LC-MS/MS) strategies. An integrated hardware and software infrastructure analyzes online LC-MS data and intelligently determines which targets to interrogate offline using a posteriori knowledge such as prior observation, identification, and degree of characterization. This platform represents a way to implement accurate mass inclusion and exclusion lists in the context of a proteome project, automating collection of high-resolution MS/MS data that cannot currently be acquired on a chromatographic time scale at equivalent spectral quality. For intact pro

SUBMITTER: Wenger CD 

PROVIDER: S-EPMC2716176 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature