Dual role of macrophage migration inhibitory factor (MIF) in human breast cancer.

ABSTRACT:

Background

Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine and mediator of acute and chronic inflammatory diseases. MIF is overexpressed in various tumours and has been suggested as a molecular link between chronic inflammation and cancer. MIF overexpression is observed in breast cancer but its causal role in the development of this tumour entity is unclear.

Methods

MIF levels in breast cancer cell lines were determined by ELISA and Western blot. CD74 was measured by Western blot, fluorescence microscopy and flow cytometry. Cell proliferation was studied by BrdU incorporation, cell adhesion by Matrigel adhesion assay, and cell invasion by migration assay through Matrigel-coated filters using the Transwell system. MIF expression in primary human breast

SUBMITTER: Verjans E 

PROVIDER: S-EPMC2716369 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature