Leukotriene pathways and in vitro adenotonsillar cell proliferation in children with obstructive sleep apnea.

ABSTRACT:

Introduction

The abundant expression of leukotrienes (LTs) and their receptors in adenotonsillar tissues of children with obstructive sleep apnea (OSA) suggest that LT antagonists could be useful in treating OSA.

Methods

The effects of LTD4 and of LT receptor antagonists zileuton, montelukast, and BAY u9773 were examined on mixed cell cultures prepared from dissociated tonsils or adenoids harvested intraoperatively from children with polysomnographically diagnosed OSA. Proliferation was assessed by (3)[H]-thymidine incorporation, and inflammatory cytokine production (tumor necrosis factor [TNF]-alpha, interleukin [IL]-6, IL-8, IL-10, and IL-12) was assessed in supernatants using enzyme-linked immunosorbent assay.

Results

LTD4 elicited dose-dependent increases in adeno

SUBMITTER: Dayyat E 

PROVIDER: S-EPMC2716539 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature