A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens.

ABSTRACT: Understanding the way in which the immune system responds to infection is central to the development of vaccines and many diagnostics. To provide insight into this area, we fabricated a protein microarray containing 1,205 Burkholderia pseudomallei proteins, probed it with 88 melioidosis patient sera, and identified 170 reactive antigens. This subset of antigens was printed on a smaller array and probed with a collection of 747 individual sera derived from 10 patient groups including melioidosis patients from Northeast Thailand and Singapore, patients with different infections, healthy individuals from the USA, and from endemic and nonendemic regions of Thailand. We identified 49 antigens that are significantly more reactive in melioidosis patients than healthy people and patients with othe

SUBMITTER: Felgner PL 

PROVIDER: S-EPMC2717108 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature