Diarylheptanoid phytoestrogens isolated from the medicinal plant Curcuma comosa: biologic actions in vitro and in vivo indicate estrogen receptor-dependent mechanisms.

ABSTRACT:

Background

Diarylheptanoids isolated from Curcuma comosa Roxb. have been recently identified as phyto estrogens. However, the mechanism underlying their actions has not yet been identified.

Objectives

We characterized the estrogenic activity of three active naturally occurring diarylheptanoids both in vitro and in vivo.

Methods

We characterized mechanisms of estrogenic action of the diarylheptanoids (3S)-1,7-diphenyl-(6E)-6-hepten-3-ol (D1), 1,7-diphenyl-(6E)-6-hepten-3-one (D2), and (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol (D3) by using a real-time polymerase chain reaction assay, a mammalian transfection model, and a uterotrophic assay in mice.

Results

All diarylheptanoids up-regulated estrogen-responsive genes in estrogen-responsive breast cancer cells (

SUBMITTER: Winuthayanon W 

PROVIDER: S-EPMC2717144 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature