Release of endogenous opioids from duodenal enteroendocrine cells requires Trpm5.

ABSTRACT:

Background & aims

Enteroendocrine cells, the largest and most diverse population of mammalian endocrine cells, comprise a number of different cell types in the gut mucosa that produce, store, and secrete small molecules, peptides, and/or larger proteins that regulate many aspects of gut physiology. Little is known about less typical endocrine cells in the intestinal mucosa that do not contain secretory granules, such as brush or caveolated cells. We studied a subset of these enteroendocrine cells in duodenum that produce several peptides, including endogenous opioids, and that also express the Trpm5 cation channel.

Methods

We studied expression patterns of Trpm5 and other molecules by immunohistochemical and enzyme-linked immunosorbent assay analyses of intestinal tissues fr

SUBMITTER: Kokrashvili Z 

PROVIDER: S-EPMC2717179 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature