DNA sequence-directed organization of chromatin: structure-based computational analysis of nucleosome-binding sequences.

ABSTRACT: The folding of DNA on the nucleosome core particle governs many fundamental issues in eukaryotic molecular biology. In this study, an updated set of sequence-dependent empirical "energy" functions, derived from the structures of other protein-bound DNA molecules, is used to investigate the extent to which the architecture of nucleosomal DNA is dictated by its underlying sequence. The potentials are used to estimate the cost of deforming a collection of sequences known to bind or resist uptake in nucleosomes along various left-handed superhelical pathways and to deduce the features of sequence contributing to a particular structural form. The deformation scores reflect the choice of template, the deviations of structural parameters at each step of the nucleosome-bound DNA from their intrins

SUBMITTER: Balasubramanian S 

PROVIDER: S-EPMC2717275 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature