Complement factor H binding by different Lyme disease and relapsing fever Borrelia in animals and human.

ABSTRACT:

Background

Borreliae employ multiple immune evasive strategies such as binding to complement regulatory proteins [factor H (fH) and factor H like-1 (FHL1)], differential regulation of surface membrane proteins, antigenic variation, and binding of plasminogen/plasmin and matrix metalloproteinases. As a complement regulatory subunit, fH serves as a cofactor for the factor I-mediated cleavage of C3b. fH binding by Borrelia has been correlated with pathogenesis as well as with host diversity. Here we show the differential binding of borrelial proteins to fH from human and animal sera.

Findings

Affinity ligand binding experiments, 2-D electrophoresis, and protein identification and peptide de novo sequencing based on mass spectrometry, revealed novel fH putative binding proteins

SUBMITTER: Bhide MR 

PROVIDER: S-EPMC2717971 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature