Optimizing TILLING and Ecotilling techniques for potato (Solanum tuberosum L).

ABSTRACT:

Background

The TILLING and Ecotilling techniques for the discovery of nucleotide polymorphisms were applied to three potato (Solanum tuberosum) cultivars treated with gamma irradiation. The three mutant cultivars tested were previously shown to exhibit salinity tolerance, an important trait in countries like Syria where increasing soil salinity is affecting agricultural production.

Findings

Three gene-specific primer pairs were designed from BAC sequence to amplify approximately 1 to 1.5 kb of gene target. One of the three primer pairs amplified a single gene target. We used this primer pair to optimize enzymatic mismatch cleavage and fluorescence DNA detection for polymorphism discovery. We identified 15 putative nucleotide polymorphisms per kilobase. Nine discovered polymo

SUBMITTER: Elias R 

PROVIDER: S-EPMC2717973 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature