PCR-based methods for the detection of L1014 kdr mutation in Anopheles culicifacies sensu lato.

ABSTRACT:

Background

Anopheles culicifacies s.l., a major malaria vector in India, has developed widespread resistance to DDT and is becoming resistant to pyrethroids-the only insecticide class recommended for the impregnation of bed nets. Knock-down resistance due to a point mutation in the voltage gated sodium channel at L1014 residue (kdr) is a common mechanism of resistance to DDT and pyrethroids. The selection of this resistance may pose a serious threat to the success of the pyrethroid-impregnated bed net programme. This study reports the presence of kdr mutation (L1014F) in a field population of An. culicifacies s.l. and three new PCR-based methods for kdr genotyping.

Methods

The IIS4-IIS5 linker to IIS6 segments of the para type voltage gated sodium channel gene of DDT and pyr

SUBMITTER: Singh OP 

PROVIDER: S-EPMC2717976 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature