Determination of interspin distance distributions by cw-ESR is a single linear inverse problem.

ABSTRACT: Cw-ESR distance measurement method is extremely valuable for studying the dynamics-function relationship of biomolecules. However, extracting distance distributions from experiments has been a highly technique-demanding procedure. It has never been conclusively identified, to our knowledge, that the problems involved in the analysis are ill posed and are best solved using Tikhonov regularization. We treat the problems from a novel point of view. First of all, we identify the equations involved and uncover that they are actually two linear first-kind Fredholm integral equations. They can be combined into one single linear inverse problem and solved in a Tikhonov regularization procedure. The improvement with our new treatment is significant. Our approach is a direct and reliable mathematica

SUBMITTER: Chiang YW 

PROVIDER: S-EPMC2718175 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature