Long-range coupling in an allosteric receptor revealed by mutant cycle analysis.

ABSTRACT: The functional coupling of residues that are far apart in space is the quintessential property of allosteric proteins. For example, in Cys-loop receptors, the gating of an intrinsic ion channel is allosterically regulated by the binding of small molecule neurotransmitters 50-60 A from the channel gate. Some residues near the binding site must have as their primary function the communication of the binding event to the gating region. These gating pathway residues are essential to function, but their identification and characterization can be challenging. This work introduces a simple strategy, derived from mutant cycle analysis, for identifying gating pathway residues using macroscopic measurements alone. In the exemplar Cys-loop receptor, the nicotinic acetylcholine receptor, a well-charac

SUBMITTER: Gleitsman KR 

PROVIDER: S-EPMC2718292 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature