Diaphragm unloading via controlled mechanical ventilation alters the gene expression profile.

ABSTRACT:

Rationale

Prolonged controlled mechanical ventilation results in diaphragmatic inactivity and promotes oxidative injury, atrophy, and contractile dysfunction in this important inspiratory muscle. However, the impact of controlled mechanical ventilation on global mRNA alterations in the diaphragm remains unknown.

Objectives

In these experiments, we used an Affymetrix oligonucleotide array to identify the temporal changes in diaphragmatic gene expression during controlled mechanical ventilation in the rat.

Methods

Adult Sprague-Dawley rats were assigned to either control or mechanical ventilation groups (n = 5/group). Mechanically ventilated animals were anesthetized, tracheostomized, and ventilated with room air for 6 or 18 h. Animals in the control group were acutely

SUBMITTER: DeRuisseau KC 

PROVIDER: S-EPMC2718415 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature