Recruited inflammatory cells mediate endotoxin-induced lung maturation in preterm fetal lambs.

ABSTRACT:

Rationale

Chorioamnionitis is paradoxically associated with a decreased incidence of respiratory distress syndrome in preterm infants. In preterm lambs, intraamniotic endotoxin and interleukin 1 (IL-1) induce lung inflammation followed by lung maturation.

Objective

To test if inflammatory cells are required to mediate induced lung maturation.

Methods

Lung inflammation was induced by intraamniotic injection of endotoxin or IL-1. Inflammatory cell recruitment to the lung was inhibited by an anti-CD18 blocking antibody given intramuscularly to the fetus. Preterm lambs were delivered at 124-d gestation (term = 150 d) 2 or 7 d after exposure to endotoxin/IL-1 or endotoxin/IL-1 + anti-CD18 antibody.

Measurements

Lung inflammation was measured by bronchoalveolar lavag

SUBMITTER: Kallapur SG 

PROVIDER: S-EPMC2718417 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature