Enhanced on-chip SERS based biomolecular detection using electrokinetically active microwells.

ABSTRACT: Here we present a novel microfluidic technique for on-chip surface enhanced Raman spectroscopy (SERS) based biomolecular detection, exploiting the use of electrokinetically active microwells. Briefly, the chip comprises of a series of microfluidic channels containing embedded microwells that, when electrically actuated, either locally attract or repulse species from solution through a combination of electrokinetic effects. We demonstrate that the approach combines the advantages of existing homogeneous (solution phase) and heterogeneous (surface phase) on-chip techniques by enabling active mixing to enhance the rate of binding between the SERS enhancers and the biomolecular targets as well as rapid concentration of the product for surface phase optical interrogation. This paper describes t

SUBMITTER: Huh YS 

PROVIDER: S-EPMC2718423 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature