A distinctive alveolar macrophage activation state induced by cigarette smoking.

ABSTRACT:

Rationale

Macrophages are believed to play a central role in emphysema based largely on data from mouse models. However, the relevance of these models to smoking-related lung disease in humans is uncertain.

Objectives

We sought to comprehensively characterize the effects of smoking on gene expression in human alveolar macrophages and to compare these with effects seen in transgenic mouse models of emphysema.

Methods

We used DNA microarrays with genomewide coverage to analyze alveolar macrophages from 15 smokers, 15 nonsmokers, and 15 subjects with asthma (disease control). Selected gene expression changes were validated by polymerase chain reaction and ELISA. Expression changes were compared with those identified by microarray analysis of interleukin-13-overexpressing

SUBMITTER: Woodruff PG 

PROVIDER: S-EPMC2718436 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature