Gene expression changes during the development of acute lung injury: role of transforming growth factor beta.

ABSTRACT:

Rationale

Acute lung injury can occur from multiple causes, resulting in high mortality. The pathophysiology of nickel-induced acute lung injury in mice is remarkably complex, and the molecular mechanisms are uncertain.

Objectives

To integrate molecular pathways and investigate the role of transforming growth factor beta (TGF-beta) in acute lung injury in mice.

Methods

cDNA microarray analyses were used to identify lung gene expression changes after nickel exposure. MAPPFinder analysis of the microarray data was used to determine significantly altered molecular pathways. TGF-beta1 protein in bronchoalveolar lavage fluid, as well as the effect of inhibition of TGF-beta, was assessed in nickel-exposed mice. The effect of TGF-beta on surfactant-associated protein B (Sftp

SUBMITTER: Wesselkamper SC 

PROVIDER: S-EPMC2718437 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature