Assessment and improvement of the Plasmodium yoelii yoelii genome annotation through comparative analysis.

ABSTRACT:

Motivation

The sequencing of the Plasmodium yoelii genome, a model rodent malaria parasite, has greatly facilitated research for the development of new drug and vaccine candidates against malaria. Unfortunately, only preliminary gene models were annotated on the partially sequenced genome, mostly by in silico gene prediction, and there has been no major improvement of the annotation since 2002.

Results

Here we report on a systematic assessment of the accuracy of the genome annotation based on a detailed analysis of a comprehensive set of cDNA sequences and proteomics data. We found that the coverage of the current annotation tends to be biased toward genes expressed in the blood stages of the parasite life cycle. Based on our proteomic analysis, we estimate that about 15% of

SUBMITTER: Vaughan A 

PROVIDER: S-EPMC2718618 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature