Quantification of pentafluorobenzyl oxime derivatives of long chain aldehydes by GC-MS analysis.

ABSTRACT: Negative ion mass spectrometric techniques, for compounds having good ionization properties, such as pentafluorobenzyl derivatives, are believed to be more sensitive than positive ion methods. Preparation of PFB oximes of fatty aldehydes from crude lipid extracts is problematic due to the release of free aldehydes from plasmalogens during derivatization. Accordingly, in these studies plasmalogens were removed by silicic acid column chromatography prior to pentafluorobenzyl derivatization. This simple purification step to remove plasmalogens is shown to facilitate the quantification of long-chain aldehydes by analysis of their pentafluorobenzyl oxime derivatives utilizing gas chromatography-mass spectrometry in the negative ion chemical ionization mode. The limit of detection for long chain

SUBMITTER: Brahmbhatt VV 

PROVIDER: S-EPMC2718725 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature