Overexpression and mislocalization of the chromosomal segregation protein separase in multiple human cancers.

ABSTRACT:

Purpose

Separase, an endopeptidase, plays a pivotal role in chromosomal segregation by separating sister chromatids during the metaphase to anaphase transition. Using a mouse mammary tumor model we have recently shown that overexpression of Separase induces aneuploidy and tumorigenesis (Zhang et al., Proc Natl Acad Sci 2008;105:13033). In the present study, we have investigated the expression level of Separase across a wide range of human tumors.

Experimental design

To examine the expression levels and localization of Separase in human tumors, we have performed immunofluorescence microscopy using human Separase antibody and tumor tissue arrays from osteosarcoma, colorectal, breast, and prostate cancers with appropriate normal controls.

Results

We show that Separase is

SUBMITTER: Meyer R 

PROVIDER: S-EPMC2718850 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature