Construction and sequence sampling of deep-coverage, large-insert BAC libraries for three model lepidopteran species.

ABSTRACT:

Background

Manduca sexta, Heliothis virescens, and Heliconius erato represent three widely-used insect model species for genomic and fundamental studies in Lepidoptera. Large-insert BAC libraries of these insects are critical resources for many molecular studies, including physical mapping and genome sequencing, but not available to date.

Results

We report the construction and characterization of six large-insert BAC libraries for the three species and sampling sequence analysis of the genomes. The six BAC libraries were constructed with two restriction enzymes, two libraries for each species, and each has an average clone insert size ranging from 152-175 kb. We estimated that the genome coverage of each library ranged from 6-9 x, with the two combined libraries of each spec

SUBMITTER: Wu C 

PROVIDER: S-EPMC2718931 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature