Global analysis of the medulloblastoma epigenome identifies disease-subgroup-specific inactivation of COL1A2.

ABSTRACT: Candidate gene investigations have indicated a significant role for epigenetic events in the pathogenesis of medulloblastoma, the most common malignant brain tumor of childhood. To assess the medulloblastoma epigenome more comprehensively, we undertook a genomewide investigation to identify genes that display evidence of methylation-dependent regulation. Expression microarray analysis of medulloblastoma cell lines following treatment with a DNA methyltransferase inhibitor revealed deregulation of multiple transcripts (3%-6% of probes per cell line). Eighteen independent genes demonstrated >3-fold reactivation in all cell lines tested. Bisulfite sequence analysis revealed dense CpG island methylation associated with transcriptional silencing for 12 of these genes. Extension of this analysis

SUBMITTER: Anderton JA 

PROVIDER: S-EPMC2719012 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature