Cellular dissection of circadian peptide signals with genetically encoded membrane-tethered ligands.

ABSTRACT:

Background

Neuropeptides regulate many biological processes. Elucidation of neuropeptide function requires identifying the cells that respond to neuropeptide signals and determining the molecular, cellular, physiological, and behavioral consequences of activation of their cognate G protein-coupled receptors (GPCRs) in those cells. As a novel tool for addressing such issues, we have developed genetically encoded neuropeptides covalently tethered to a glycosylphosphatidylinositol (GPI) glycolipid anchor on the plasma membrane ("t-peptides").

Results

t-peptides cell-autonomously induce activation of their cognate GPCRs in cells that express both the t-peptide and its receptor. In the neural circuit controlling circadian rest-activity rhythms in Drosophila melanogaster, rhythmic

SUBMITTER: Choi C 

PROVIDER: S-EPMC2719018 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature