Secretory Transactivating Transcription-apoptin fusion protein induces apoptosis in hepatocellular carcinoma HepG2 cells.

ABSTRACT:

Aim

To determine whether SP-TAT-apoptin induces apoptosis and also maintains its tumor cell specificity.

Methods

In this study, we designed a secretory protein by adding a secretory signal peptide (SP) to the N terminus of Transactivating Transcription (TAT)-apoptin (SP-TAT-apoptin), to test the hypothesis that it gains an additive bystander effect as an anti-cancer therapy. We used an artificial human secretory SP whose amino acid sequence and corresponding cDNA sequence were generated by the SP hidden Markov model.

Results

In human liver carcinoma HepG2 cells, SP-TAT-apoptin expression showed a diffuse pattern in the early phase after transfection. After 48 h, however, it translocated into the nuclear compartment and caused massive apoptotic cell death, as determine

SUBMITTER: Han SX 

PROVIDER: S-EPMC2719227 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature