Phenotypic screen of early-developing larvae of the blood fluke, schistosoma mansoni, using RNA interference.

ABSTRACT: RNA interference (RNAi) represents the only method currently available for manipulating gene-specific expression in Schistosoma spp., although application of this technology as a functional genomic profiling tool has yet to be explored. In the present study 32 genes, including antioxidants, transcription factors, cell signaling molecules and metabolic enzymes, were selected to determine if gene knockdown by RNAi was associated with morphologically definable phenotypic changes in early intramolluscan larval development. Transcript selection was based on their high expression in in vitro cultured S. mansoni primary sporocysts and/or their potential involvement in developmental processes. Miracidia were allowed to transform to sporocysts in the presence of synthesized double-stranded RNAs (ds

SUBMITTER: Mourao MM 

PROVIDER: S-EPMC2719580 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature