Identification of potential therapeutic targets in human head & neck squamous cell carcinoma.

ABSTRACT:

Background

Human head and neck squamous cell carcinoma (HNSCC) is an aggressive and recurrent malignancy. Identification of unique or overexpressed cell-associated or cell surface antigens is critical for diagnosis and development of cancer vaccines and targeted therapies for HNSCC. We have used high throughput microarray technology to search for candidate targets in HNSCC.

Methods

Gene expression profiling in 17 HNSCC tumors and 3 normal tonsil tissues was performed by microarray. QRT-PCR analysis was performed to validate the microarray results. The five candidate genes were further characterized by immunohistochemical technique in surgical samples and tissue arrays.

Results

A total of 192 up-regulated genes at statistical significance of p < 0.01 and log2 ratio > o

SUBMITTER: Han J 

PROVIDER: S-EPMC2719634 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature