Characterization of a novel telomerase-immortalized human endometrial stromal cell line, St-T1b.

ABSTRACT:

Background

Coordinated differentiation of the endometrial compartments in the second half of the menstrual cycle is a prerequisite for the establishment of pregnancy. Endometrial stromal cells (ESC) decidualize under the influence of ovarian progesterone to accommodate implantation of the blastocyst and support establishment of the placenta. Studies into the mechanisms of decidualization are often hampered by the lack of primary ESC. Here we describe a novel immortalized human ESC line.

Methods

Primary ESC were immortalized by the transduction of telomerase. The resultant cell line, termed St-T1b, was characterized for its morphological and biochemical properties by immunocytochemistry, RT-PCR and immunoblotting. Its progestational response was tested using progesterone and

SUBMITTER: Samalecos A 

PROVIDER: S-EPMC2719639 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature