Virtual terminator nucleotides for next-generation DNA sequencing.

ABSTRACT: We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.

SUBMITTER: Bowers J 

PROVIDER: S-EPMC2719685 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature