The carboxyl terminus of Brca2 links the disassembly of Rad51 complexes to mitotic entry.

ABSTRACT:

Background

The Rad51 recombinase assembles on DNA to execute homologous DNA recombination (HR). This process is essential to repair replication-associated genomic lesions before cells enter mitosis, but how it is started and stopped during the cell cycle remains poorly understood. Rad51 assembly is regulated by the breast cancer suppressor Brca2, via its evolutionarily conserved BRC repeats, and a distinct carboxy (C)-terminal motif whose biological function is uncertain. Using "hit-and-run" gene targeting to insert single-codon substitutions into the avian Brca2 locus, we report here a previously unrecognized role for the C-terminal motif.

Results

We show that the avian C-terminal motif is functionally cognate with its human counterpart and identify point mutations that eit

SUBMITTER: Ayoub N 

PROVIDER: S-EPMC2719694 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature