Muscle-specific calpain is localized in regions near motor endplates in differentiating lobster claw muscles.

ABSTRACT: Calpains are Ca2+-dependent proteinases that mediate protein turnover in crustacean skeletal muscles. We used an antibody directed against lobster muscle-specific calpain (Ha-CalpM) to examine its distribution in differentiating juvenile lobster claw muscles. These muscles are comprised of both fast and slow fibers early in development, but become specialized into predominantly fast or exclusively slow muscles in adults. The transition into adult muscle types requires that myofibrillar proteins specific for fast or slow muscles to be selectively removed and replaced by the appropriate proteins. Using immunohistochemistry, we observed a distinct staining pattern where staining was preferentially localized in the fiber periphery along one side of the fiber. Immunolabeling with an antibody di

SUBMITTER: Medler S 

PROVIDER: S-EPMC2719716 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature