Post-transcriptional processing generates a diversity of 5'-modified long and short RNAs.

ABSTRACT: The transcriptomes of eukaryotic cells are incredibly complex. Individual non-coding RNAs dwarf the number of protein-coding genes, and include classes that are well understood as well as classes for which the nature, extent and functional roles are obscure. Deep sequencing of small RNAs (<200 nucleotides) from human HeLa and HepG2 cells revealed a remarkable breadth of species. These arose both from within annotated genes and from unannotated intergenic regions. Overall, small RNAs tended to align with CAGE (cap-analysis of gene expression) tags, which mark the 5' ends of capped, long RNA transcripts. Many small RNAs, including the previously described promoter-associated small RNAs, appeared to possess cap structures. Members of an extensive class of both small RNAs and CAGE tags were di

SUBMITTER: Affymetrix ENCODE Transcriptome Project 

PROVIDER: S-EPMC2719882 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature