Rapid DNA methylation changes after exposure to traffic particles.

ABSTRACT:

Rationale

Exposure to particulate air pollution has been related to increased hospitalization and death, particularly from cardiovascular disease. Lower blood DNA methylation content is found in processes related to cardiovascular outcomes, such as oxidative stress, aging, and atherosclerosis.

Objectives

We evaluated whether particulate pollution modifies DNA methylation in heavily methylated sequences with high representation throughout the human genome.

Methods

We measured DNA methylation of long interspersed nucleotide element (LINE)-1 and Alu repetitive elements by quantitative polymerase chain reaction-pyrosequencing of 1,097 blood samples from 718 elderly participants in the Boston area Normative Aging Study. We used covariate-adjusted mixed models to account fo

SUBMITTER: Baccarelli A 

PROVIDER: S-EPMC2720123 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature