Smoking-related genomic signatures in non-small cell lung cancer.

ABSTRACT:

Rationale

Tobacco smoking is responsible for 85% of all lung cancers. To further our understanding of the molecular pathogenesis of lung cancer, we determined whether smoking history leads to the emergence of specific genomic alterations found in non-small cell lung cancer (NSCLC).

Objectives

To identify gene copy number alterations in NSCLCs associated with smoking history or DNA repair capacity.

Methods

Seventy-five NSCLCs were selected for this study from patients with current, none, or past smoking history, including pack year information. Tissue sections were microdissected, and DNA was extracted, purified, and labeled by random priming before hybridization onto bacterial artificial chromosome (BAC) arrays. Normalized ratios were correlated with smoking history a

SUBMITTER: Massion PP 

PROVIDER: S-EPMC2720147 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature