Induction of apoptosis in human cancer cells by candidaspongiolide, a novel sponge polyketide.

ABSTRACT:

Background

Candidaspongiolide (CAN), a novel polyketide from a marine sponge, is the active component of a mixture that was found to be potently cytotoxic in the National Cancer Institute's 60-cell-line screen.

Methods

Effects of CAN on U251 glioma and HCT116 colorectal cancer cells and on normal fibroblasts were assessed using radiolabeling studies to measure protein synthesis, clonogenic assays to measure cell survival, flow cytometry of annexin V- and propidium iodide-stained cells to measure apoptosis, and western blots in the presence or absence of specific inhibitors to assess accumulation and phosphorylation of potential downstream target proteins.

Results

CAN inhibited protein synthesis and potently induced apoptosis in both U251 and HCT116 cells, the latter i

SUBMITTER: Trisciuoglio D 

PROVIDER: S-EPMC2720712 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature