Quantitation of Aurora kinase A gene copy number in urine sediments and bladder cancer detection.

ABSTRACT:

Background

Chromosome missegregation and the resulting aneuploidy is a common change in neoplasia. The Aurora kinase A (AURKA) gene, which encodes a key regulator of mitosis, is frequently amplified and/or overexpressed in cancer cells, and the level of AURKA amplification is associated with the level of aneuploidy. We examined whether AURKA gene amplification is a biomarker for the detection of bladder cancer.

Methods

The effect of ectopic expression of Aurora kinase A (AURKA) using an adenoviral vector in simian virus 40-immortalized urothelial cells (SV-HUC) on centrosome multiplication and chromosome copy number was measured in vitro by immunofluorescence and fluorescence in situ hybridization (FISH), respectively. The FISH test was also used to examine AURKA gene copy n

SUBMITTER: Park HS 

PROVIDER: S-EPMC2720731 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature