Lysophosphatidic acid receptors determine tumorigenicity and aggressiveness of ovarian cancer cells.

ABSTRACT:

Background

Lysophosphatidic acid (LPA) acts through the cell surface G protein-coupled receptors, LPA1, LPA2, or LPA3, to elicit a wide range of cellular responses. It is present at high levels in intraperitoneal effusions of human ovarian cancer increasing cell survival, proliferation, and motility as well as stimulating production of neovascularizing factors. LPA2 and LPA3 and enzymes regulating the production and degradation of LPA are aberrantly expressed by ovarian cancer cells, but the consequences of these expression changes in ovarian cancer cells were unknown.

Methods

Expression of LPA1, LPA2, or LPA3 was inhibited or increased in ovarian cancer cells using small interfering RNAs (siRNAs) and lentivirus constructs, respectively. We measured the effects of changes in

SUBMITTER: Yu S 

PROVIDER: S-EPMC2720766 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature